当社グループは 3,000 以上の世界的なカンファレンスシリーズ 米国、ヨーロッパ、世界中で毎年イベントが開催されます。 1,000 のより科学的な学会からの支援を受けたアジア および 700 以上の オープン アクセスを発行ジャーナルには 50,000 人以上の著名人が掲載されており、科学者が編集委員として名高い
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700 ジャーナル と 15,000,000 人の読者 各ジャーナルは 25,000 人以上の読者を獲得
Che-Ping Lin, Yueh-Che Sung and Ging-Ho Hsiue
This study demonstrates new ternary polyion gene carriers, based on the PH.-responsive diblock copolymer poly(2-ethyl-oxazoline)-block-poly(methacrylic acid) (PEOz-b-PMAA), and the branched-poly (ethylenimine) (B-PEI). This study complexes the plasmid DNA with B-PEI and further with PEOz-b-PMAA to obtain ternary polyplexes (DNA/B-PEI/PEOz-b-PMAA). PMAA were partially dissociated under neutral PH. with a negative charge, to attach to the positive charge surface of the B-PEI/DNA polyplex. The ternary polyplexes will also desorb and return to the original pre-poly complex to help gene release after cell uptake due to PMMA become neutral charge under an acid environment in endosome. The ternary polyplexes showed suitable mean particle size, low cytotoxicity, and acceptable transfection at PH 7.4 because of shielding of B-PEI by PEOz-b-PMAA. TEM morphology showed that the stable core-shell structure of ternary polyplexes at PH. 7.4 collapsed and released plasmid at PH. 5. Observations of cell uptake of the B-PEI/DNA polyplex and ternary polyplexes by CLSM revealed that ternary polyplexes started to accumulate after 3 h incubation and accumulated significantly after 6 h. In conclusion, the ternary polyplex improves cytotoxicity of the single B-PEI/DNA polyplex, and presents a PH.-responsive behavior to enhance gene escape from the polyplex. The ternary polyplex constitutes a useful approach for gene carrier design.