English 
Spanish 
Chinese 
Russian 
German 
French 
Portuguese 
Hindi 当社グループは 3,000 以上の世界的なカンファレンスシリーズ 米国、ヨーロッパ、世界中で毎年イベントが開催されます。 1,000 のより科学的な学会からの支援を受けたアジア および 700 以上の オープン アクセスを発行ジャーナルには 50,000 人以上の著名人が掲載されており、科学者が編集委員として名高い
。オープンアクセスジャーナルはより多くの読者と引用を獲得
700 ジャーナル と 15,000,000 人の読者 各ジャーナルは 25,000 人以上の読者を獲得
インデックス付き
- Google スカラー
- レフシーク
- ハムダード大学
- エブスコ アリゾナ州
- パブロン
- ICMJE
役立つリンク
オープンアクセスジャーナル
このページをシェアする
抽象的な
Anti-Tumoral Effects of Natural Killer Cells Differentiated In Vitro from Cord-Blood Hematopoietic Stem Cells on Umbilical Cord Mesenchymal Stem Cells against Glioblastoma
Ege Cubuk, Latife Merve Oktay, Hilal Kabadayi Ensarioglu, H. Seda Vatansever, Gulinnaz Ercan
Purpose: Glioblastoma is the most malignant primary adult brain tumor with a poor prognosis; therefore, novel therapies are needed. Natural Killer (NK) cells are promising candidates for immunotherapy because of their ability to eliminate tumor cells without prior sensitization. An inadequate number of NK cells remain a major obstacle. The primary purpose of this study was to develop NK cells from Cord Blood Hematopoietic Stem Cells (CB-HSCs) on Umbilical Cord Mesenchymal Stem Cells (UC-MSCs) feeder layer and analyse cytotoxic potential of NK cells against glioblastoma in vitro.
Methods: UC-MSCs were treated with mitomycin-C to be used as a feeder layer. CB-HSCs were co-cultured with UC-MSCs and differentiated into NK cells by using medium containing cytokines such as thrombopoietin, Flt3- ligand, stem cell factor, IL-6, IL-7, IL-15 and IL-2. NK cells were characterized by immunocytochemistry. The in vitro cytotoxic effect of NK cells on T98-glioblastoma cells was determined by annexin V‑fluorescein isothiocyanate/ propidium iodide staining followed by flow cytometric analysis. qRT-PCR was performed to measure gene expression levels of KRAS, TP53, TGFBR2 and NANOG in glioblastoma cells after treatment with NK cells.
Results: NK cells were successfully differentiated from CB-HSCs on the UC-MSC feeder layer with the strong expression of cytotoxic receptors after 6 weeks. They demonstrated potent cytotoxicity against glioblastoma in vitro. Additionally, the KRAS oncogene expression in glioblastoma cells decreased upon co-culture with NK cells.
Conclusion: NK cells differentiated from CB-HSCs on UC-MSC feeder-layer were capable of eliminating glioblastoma cells via apoptosis in vitro and warrant further investigation in vivo and clinical settings.