がん予防の進歩

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Calcium-activated potassium channels as potential early markers of cervical cancer

Javier Camacho

Cervical cancer is a major cause of cancer death in women in developing countries. Thus, novel early markers
and therapeutic targets are urgently needed. Ion channels have gained great interest as tumor markers for different
malignancies including cervical cancer. Actually, some years ago, we suggested Kv10.1 channel as cervical
cancer early marker. Here, we studied the expression of another potassium channel, namely, the calcium-activated
potassium channel KCa1.1 (KCNMA1) in cervical cancer models. Transgenic mice expressing the E7 oncogene of
human papilloma virus and non-transgenic mice were treated with estradiol pellets during three or six months to
induce cervical lesions. Human biopsies from patients with either noncancerous, low- or high-grade intra-epithelium
lesions or cervical cancer were also studied. mRNA and protein expression were studied by real-time RT-PCR and
immunochemistry, respectively. Cervical dysplasia and cervical cancer were observed only in the transgenic mice
treated with estradiol for three and six months, respectively. Estradiol treatment increased KCa1.1 mRNA and protein
expression in both transgenic and non-transgenic mice. However, the highest levels were observed in the transgenic
mice with cervical cancer. Human biopsies form non-cancerous cervix did not display KCa1.1 protein expression.
However, increased KCa1.1 protein expression was observed in the rest of the human biopsies, we observed that the
higher the grade of the lesion, the stronger the KCa1.1 immune staining. These results suggest KCa1.1 channel as
potential early cervical cancer marker.