薬学および生命科学における研究開発の国際ジャーナル

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CHARACTERIZATION OF HIGH ACTIVITY DRUG-STIMULATED ATPase OF MULTIDRUG RESISTANT LEISHMANIA DONOVANI AMASTIGOTE CELLS

Chowdhury S, Roy A, Kar N, Chakraborty S, Bera T

Background: The emergence of drug resistance and the limited knowledge of the mechanisms by which the parasites acquire resistance are the major obstracle for the control of leishmaniasis.
Methods: The present investigation is aimed to study multidrug resistance related ATPase activity in in vitro generated sodium stibogluconate (SSG) and paramomycin (PMM) resistant Leishmania donovani amastigote permeablized by digitonin.
Results: The results of the multidrug resistance related ATPase activity in SSG and PMM resistant L.donovani amastigotes revealed that ATPase activities were strongly stimulated by resistance causing drugs, and these ATPase stimulation activities had broad specificities.
Conclusion: Generation of drug resistance in L. donovani amastigotes leads to concurrent development of drug-stimulated ATPase activity.