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Development and Validation of a Method for Densitometric Analysis of 6-Gingerol in Herbal extracts and Polyherbal Formulation

P. S. Jain, A. U. Tatiya, S. A. Bagul and S. J. Surana

A simple, selective, precise densitometric HPTLC method for analysis of 6-Gingerol both in chloroform extract of Zingiber officinalis and in laxiwin formulation has been developed and validated. The method employed HPTLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The phytoconstituent, 6-Gingerol was separated with mobile phase toluene: ethyl acetate (7:3 %, v/v). A compact band was obtained for 6-gingerol at R F 0.33±0.02. Densitometric analysis of 6-gingerol was carried out in the absorbance mode at 254 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r 2 = 0.9981 with respect to peak area in the concentration range 150–900 ng per spot. The mean value of slope and intercept were 4.103 and 398.6 with respect to peak area. The method was validated for precision, recovery and robustness. The limits of detection and quantitation were 24.13 and 63.83 ng per spot, respectively. This novel Statistical analysis proves that the method is repeatable and selective for the analysis of 6-Gingerol in extract and in pharmaceutical formulations.