English 
Spanish 
Chinese 
Russian 
German 
French 
Portuguese 
Hindi 当社グループは 3,000 以上の世界的なカンファレンスシリーズ 米国、ヨーロッパ、世界中で毎年イベントが開催されます。 1,000 のより科学的な学会からの支援を受けたアジア および 700 以上の オープン アクセスを発行ジャーナルには 50,000 人以上の著名人が掲載されており、科学者が編集委員として名高い
。オープンアクセスジャーナルはより多くの読者と引用を獲得
700 ジャーナル と 15,000,000 人の読者 各ジャーナルは 25,000 人以上の読者を獲得
インデックス付き
- Google スカラー
- レフシーク
- ハムダード大学
- エブスコ アリゾナ州
- パブロン
- ICMJE
役立つリンク
オープンアクセスジャーナル
このページをシェアする
抽象的な
Enhancement of Cisplatin-Induced Apoptosis of Human Oral Squamous Cell Carcinoma by FBXW7
Qi Yang, Yang Sun, Bo Qiu, Huanhuan Zhao
Background: About one-third of Oral Squamous Cell Carcinoma (OSCC) patients have a risk of occurrence and chemo resistance, making the survival abysmal. We aim to evaluate the role of F-Box/WD repeat-containing protein 7 (FBXW7) to further develop efficient treatment of chemo resistant OSCC.
Methods: FBXW7 overexpression was induced by a lent viral vector, Lv-FBXW7 or lv-NC (non-coding control) and overexpression efficiency was assessed using quantitative real-time PCR (qRT-PCR) and western blot of FBXW7. Cell viability was measured using MTT assay. The effects of FBXW7 overexpression on cell migration and invasion was evaluated by the colony formation assay and Matrigel assay. Apoptosis of cells with lv-FBXW7 transfection was measured by qRT-PCR and western blot analyses of BAX, BAK, MCL1 and BCL2 expression. Growth rate and Cisplatin sensitivity of CAL27 xenografts with or without FBXW7 overexpression was monitored. Ki-67 and PCMA levels, which are biomarkers of intra-tumoral apoptosis, BAX, MCL1, Beclin1, LC3I and II, which are autophagy biomarkers, were assessed.
Results: Transfection of lv-FBXW7 in SCC9 and CAL27 cells resulted in increased sensitivity to Cisplatin treatment, as evidenced by slower cell proliferation, lower colony formation and invasion, higher apoptosis and autophagy, compared to those transfected with lv-NC. Mice with CAL27 xenografts overexpressing FBXW7 also demonstrated slower tumor growth and up regulation in Ki067 and PCNA. Tumors also showed higher apoptosis and autophagy activities.
Conclusion: FBXW7 overexpression was herein shown to effectively sensitize OSCC cells to Cisplatin treatment in vitro and in vivo, potentiating FBXW7 transduction as a new treatment strategy for OSCC.