臨床薬理学および生物薬剤学

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Inhibitory Potential of Yucca Gloriosa L. Extract and Isolated Gloriosaol Isomeric Mixture on Ovalbumin Induced Airway Hyperresponsiveness in Balb/C Mice

Saurabh Gupta, Basavan Duraiswamy, Satish Kumar Muthureddy Nataraj, Rama Satyanarayana Raju K, Babu UV, Sharath Kumar LM, Omji Porwal and Renu Gupta

Yucca gloriosa (Agavaceae) is used in Indian traditional medicine as an antiallergic drug. The ethanol extract was prepared with aerial parts of Yucca gloriosa (YGE). Gloriosaol isomeric mixtures (GLM) are isolated from the extract, structurally confirmed FIA-MS/MS. Both YGE and GLM are evaluated against ovalbumin-induced airway hyperresponsiveness (AHR) in Balb/C mice. The test drugs (GLM or YGE or Dexamethasone) are administered p.o. prior to challenge with aerosolized 2.5% w/v ovalbumin. Total and differential leucocytes count, Nitrite (NO2), Nitrate (NO3), Tumor necrosis factor- α (TNF-α), Interlukin-6 (IL-6), and Interlukin-13 (IL-13) are estimated in bronchoalveolar lavage fluid (BALF). Similarly, Myeloperoxidase (MPO) and Malonaldehyde (MDA) are estimated in lungs. The results reveal a significant increase in total and differential leucocytes counts, NO2, NO3, TNF-α, IL-6, and IL-13 in ovalbumin induced AHR. However, these parameters are significantly decreased in YGE and GLM treated mice at test doses (YGE 100 & 200 mg/kg and GLM 30 mg/kg). Similar observations are recorded for Myeloperoxidase (MPO) and Malonaldehyde (MDA) in lungs. Pro inflammatory mediators are (TNF-α, IL-6, and IL-13) known that responsible for AHR. Histopathology reveled justify the effectiveness. The present investigations suggest both YGE and GLM are interesting molecules for the further research the treatment of asthma, with an approach through pro-inflammatory inhibitory pathway.