生化学と生理学: オープンアクセス

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Integrins Contributes to Innate Immune Response in Pelteobagrus Fulvidraco

Ming-Ming Han, Jian-Guo Lu, Shang-Bin, Li-Na-Peng, Shahid Mahboob, Khalid A Al-Ghanim and Xiao-Wen Sun

Integrins - β are highly conserved pleiotropic cytokine. In this study, we cloned the full - length cDNA sequence of yellow catfish Pelteobagrus fulvidraco integrin - β1 and integrin - β3 gene. The Integrin - β1 cDNA is 2816 bp in length and encodes a protein of 461 amino acids. Integrin - β3 cDNA is 1713 bp in length and encodes 479 amino acids. Integrins - β were classified strictly with the same type of proteins by phylogenetic analysis. Stegastes partitus integrins - β was classified on the same phylogenetic branch compared with other fish. Tissue transcription profile analysis indicated that, in the healthy P. fulvidraco, integrins - β mRNA was predominantly expressed in the liver and brain, follow by gill and spleen. The P. fulvidraco integrins - β shares high homology with the Cypriniformes Danio rerio (83% ~87%). Expression of the two Integrins - β transcripts showed significant expression changes in 4 h after infection with Flavobacterium columnare, which has modulation of expression continuing up to 3 d following pathogen exposure. Both of the integrin - β genes were significantly up - regulated at 24 h in spleen and liver after infection tissue. Integrin - β3 showed the most sensitive to F. columnare, suppressing expression - 6.34 - fold at 24h. This informations elucidates the functions of individual integrins - β genes in regards to pathogen recognition, binding, and their larger immune context.