伝統医学と臨床自然療法

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In vitro Evaluation of Centratherum anthelminticum Seeds for Antinephrolithiatic Activity

Varsha J. Galani and Rital R. Panchal

Antinephrolithiatic activity of 70% methanolic extract of Centratherum anthelminticum seeds (CAE) was evaluated in vitro on nucleation and aggregation of calcium oxalate crystallization. Calcium oxalate crystallization was induced by the addition of 0.01 M sodium oxalate solution in synthetic urine. The effect of CAE (100, 200, 300, 400, 600, 800 and 1000 μg/ml) was studied by the measurement of turbidity in presence or absence of extract at 620 nm of a spectrophotometer. The rates of nucleation and aggregation were evaluated by comparing the turbidity of a control system with that of one exposed to the extract. Crystals in the urine were also analysed by light microscopy. From photomicrograph, it is confirmed that CAE inhibited the nucleation of calcium oxalate crystals, decreasing their number
and size. Also percentage inhibition of crystals aggregation increased as the concentration of CAE increased. The results of the present study indicated that 70% methanolic extract of C. anthelminticum seeds has the higher capacity to inhibit the crystal formation and aggregation. These suggested possible antinephrolithiatic activity of C. anthelminticum seeds against calcium oxalate stones.