海洋科学: 研究開発

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Isolation and Identification of A Novel Aporphine Alkaloid SSV, An Antitumor Antibiotic from Fermented Broth of Marine Associated Streptomyces sp. KS1908

Sunanda Kadiri, Nagendra Sastry Yarla and Siddaiah Vidavalur

A marine actinomycete, Streptomyces sp. KS1908 was isolated from a marine sediment sample collected in
the Bay of Bengal, India and identified based on morphological, cultural, physiological, biochemical characteristics,along with the cell wall analysis and 16S rRNA gene sequence analysis. Strain KS1908 has more similarities with Streptomyces albus but some variations were observed. A novel aporphine alkaloid SSV was isolated according to bioactivity guided fractionation of fermented broth through solvent extraction and chromatography. Chemical structure of the aporphine alkaloid SSV was elucidated on the basis of spectroscopic analysis, including two-dimensional (2D) NMR and HR-ESI-MS data. Aporphine alkaloid SSV showed significant antibacterial, antifungal activity and also possesses considerable anticancer activity against human larynx (HEp-2), cervical (HeLa), leukemia HL-60 and MCF-7 breast cancer cell lines.