薬学および生命科学における研究開発の国際ジャーナル

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Metabolomic Profiling of Electrolytes, Lipid and Steroids in Saliva during Menstrual Cycle

Subbarayalu Alagendran, Mohanadoss Ponraj, M. Rajasekaran, Gaby Fernandez Saarvedra, Liswaniso Chiyomba

During reproductive cycle, women’s do not show the consequent cyclical changes that would be an indicator of ovulation, therefore it is important to extend a method to identify the time of ovulation. Ovulation is such a decisive physiological process that its noninvasive detection based on salivary biochemicals has various advantages in human diagnosis. The present study is proposed to identify the ovulatory-specific biochemicals in saliva in order to identify ovulation phase. The rapid test and noninvasive study made in salivary biochemical’s as electrolytes, profile of lipids and putative hormones in saliva during pre-ovulatory, ovulatory and post-ovulatory phases of menstrual cycle. Saliva was collected from 120 menstruating women during preovulatory, ovulatory and postovulatory phases and electrolytes, lipid profile and hormones were analyzed. In particular electrolytes like sodium, potassium, lipids like total cholesterol, phospholipids, steroid hormones such as estrogen, progesterone and their derivatives were analyzed. Salivary electrolytes and lipid profiles in saliva increased significantly (<0.01) during the phase of ovulation at the time of LH surge while comparing to pre- and post-ovulatory phase also measured by ferning pattern. Estrogen and its derivative increased significantly (<0.01) during the ovulatory phase as compared to preovulatory phase, while progesterone and its conjugates increased significantly (<0.01) during post-ovulatory phase when compared to other two phases. As a conclusion, electrolytes, lipid profile and estrogen were found to be increased during ovulatory phase in normal menstruating women.