English 
Spanish 
Chinese 
Russian 
German 
French 
Portuguese 
Hindi 当社グループは 3,000 以上の世界的なカンファレンスシリーズ 米国、ヨーロッパ、世界中で毎年イベントが開催されます。 1,000 のより科学的な学会からの支援を受けたアジア および 700 以上の オープン アクセスを発行ジャーナルには 50,000 人以上の著名人が掲載されており、科学者が編集委員として名高い
。オープンアクセスジャーナルはより多くの読者と引用を獲得
700 ジャーナル と 15,000,000 人の読者 各ジャーナルは 25,000 人以上の読者を獲得
インデックス付き
- 索引コペルニクス
- Google スカラー
- Jゲートを開く
- レフシーク
- ハムダード大学
- エブスコ アリゾナ州
- OCLC-WorldCat
- パブロン
- ユーロパブ
- ICMJE
役立つリンク
オープンアクセスジャーナル
このページをシェアする
抽象的な
Multilocus Sequence Typing and Molecular Detection of Phenol-soluble Modulin in Biofilm-positive Staphylococcus epidermidis Isolated from Paediatric Blood Culture
Grace A John- Ugwuanya, Stephen K Obaro, Busayo O Olayinka, Josiah A Onaolapo, Fatimah Hassan Hanga, Huda Munir and Paul D Fey
Aim: Staphylococcus epidermidis is a significant coagulase-negative staphylococci obtained from blood culture samples. However, there is limited information about phenol-soluble modulin (PSM), which is associated with virulence in S. epidermidis and its genetic relatedness in Nigeria. This study observed the presence of phenolsoluble modulin mec (psm-mec) gene and the multilocus sequence typing (MLST) of biofilm-positive Staphylococcus epidermidis (BPSE).
Method: Twenty-two biofilm-positive S. epidermidis isolates obtained from paediatric blood culture at three hospitals in north-west and north-central Nigeria were evaluated for the molecular detection of the psm-mec gene using conventional polymerase chain reaction (PCR). The biofilm formation was previously assessed by molecular detection of the intercellular adhesion (icaA) gene and the methicillin resistance using cefoxitin disk agar diffusion. Internal fragments of the respective seven housekeeping genes was sequenced for 21 BPSE strains and matched with the central MLST database.
Results: Out of 22 BPSE, only 4.5% had the psm-mec gene and it was methicillin resistant. About 91% methicillin resistance was observed among the psm-mec negative BPSE strains. Twenty-one BPSE strains were sequence type 1 (ST1) based on the MLST analysis of the seven housekeeping genes.
Conclusion: Considering the very low presence of psm-mec gene, the BPSE may be carrying the ß-type PSMs related to biofilm formation and dissemination, and not the cytolytic a-type PSMs common in the aggressive form of S. epidermidis. Phenol-soluble modulin methicillin resistance island-encoded peptide toxin is involved in sepsis related methicillin-resistant S. epidermidis. The strains are genetically related to each other.