バイオレメディエーションと生分解のジャーナル

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Optimization of Cellulase Enzyme Production from Sugarcane Pressmud Using Oyster Mushroom - Pleurotus Sajor-Caju by Solid State Fermentation

Nitin Prakash Pandit and Sanjiv Kumar Maheshwari

Cellulose is often a major component of different waste streams from various agriculture and sugarcane industries. Bioconversion of cellulosic components into fermentable sugars has currently been accomplished with the help of the microbial enzyme, cellulase (Endo-β-1, 4-glucanase, Exo-β-1, 4-glucanase and β-glucosidase). In this study, Pleurotus sajor-caju was tested for cellulase production by solid state fermentation (SSF) using sugarcane pressmud as substrate. Production rate of enzyme were optimized with different parameters like pretreatment of substrate with NaOH, HCl, H 2 O 2 and NaOH + H 2 O 2 (1-5%) solutions, thickness of the substrate (0.4-2.0 cm), incubation times (1-16 days), optimum pH (2.0-8.0) and temperature (15 ÌŠC-40 ÌŠC). The highest activities of Endo-β-1, 4-glucanase, Exo-β-1, 4-glucanase and β-glucosidase were obtained as follows: 13.94, 8.84 and 12.29 Units (μmole of glucose released/min/g substrate), respectively, for the pretreatment of pressmud with 2% (H 2 O 2 +NaOH); 18.37, 12.09 and 16.47 Units (μmole of glucose released/min/g substrate), respectively, when the thickness of the substrate was 0.8 cm (10 g); 15.51, 11.09 and 13.99 Units (μmole of glucose released/min/g substrate), respectively, on 10 th day of incubation; 17.65, 13.49 and 14.62 Units (μmole of glucose released/min/g substrate), respectively, at 5 pH and 18.98, 13.63 and 18.54 Units (μmole of glucose released/min/g substrate), respectively, at 25 ÌŠC. The optimum culture conditions for the production of cellulases by Pleurotus sajor-caju were 10 days of incubation time at 25 ÌŠC and pH 5.0 when presmud was pretreated with H 2 O 2 +NaOH (2%) and the thickness of the substrate was 0.8 cm (10 g) under solid state fermentation.