バイオレメディエーションと生分解のジャーナル

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Optimization of Phenol Degradation Using Pseudomonas aeruginosa (MTCC 7814) by Plackett-Burman Design and Response Surface Methodology

Pandimadevi M, Venkatesh Prabhu M, Vinod Kumar V

In the present study, statistical screening and optimization of phenol degradation was done by Pseudomonas Aeruginosa (MTCC 7814), which can utilize phenol as a sole carbon and energy source. Nine medium variables Phenol, K₂HPO₄, K₂H₂PO₄, MgSO₄, (NH₄)₂SO₄, MnSO₄, FeSO₄, NaCl and H₃BO₃ were screened by Plackett-Burman (PB) method. K₂HPO₄, (NH₄)₂SO₄, MnSO₄ and phenol were significant in PB method. Central composite design (CCD) and Response Surface Methodology (RSM) were applied to optimize the significant variables identified from the PB experiment. Statistical analysis of the experimental results showed optimal values were found to be KH₂PO₄ 0.025 g/L, (NH₄)₂SO₄ 0.45 g/L, MnSO4 0.05 g/L and phenol 1 g/L with maximum phenol degradation of 83.86%. Maximum phenol degradation of 81.62% was observed in the validation experiment. This experimental result explained the model was fitted 97.32 % as compare with the result predicted by response surface method. This study indicated the excellent ability of Pseudomonas Aeruginosa to degrade phenol of high concentration.