当社グループは 3,000 以上の世界的なカンファレンスシリーズ 米国、ヨーロッパ、世界中で毎年イベントが開催されます。 1,000 のより科学的な学会からの支援を受けたアジア および 700 以上の オープン アクセスを発行ジャーナルには 50,000 人以上の著名人が掲載されており、科学者が編集委員として名高い
。オープンアクセスジャーナルはより多くの読者と引用を獲得
700 ジャーナル と 15,000,000 人の読者 各ジャーナルは 25,000 人以上の読者を獲得
Cristina Cifuentes, Sigrid Mennickent and Marta De Diego
A thin-layer chromatographic (HPTLC) method for quantification of topiramate in human breast milk was developed using liquid –liquid extraction with n-hexane and methanol as extraction solvents, fluorescence activation with ninhidrine (1% ethanolic solution) and chlorpromazine as internal standard.
Thin-layer chromatographic separation was performed on precoated silica gel F 254 HPTLC plates using a mixture of toluene: ethanol (25:10, v/v), as mobile phase. Densitometric detection was done at 326 nm. The method was validated for linearity, precision, selectivity, LOD and LOQ, and accuracy. Linear calibration curves in the range of 0.30 to 50.00 µg/mL showed correlation coefficient of 0.991. The intra-assay and inter-assay precision, expressed as the relative standard deviation (RSD), were in the range of 3.04% - 3.14% (n=3) and 1.81%-4.10% (n=9), respectively. The limit of detection was 0.24 µg/mL, and the limit of quantification was 0.30 µg/mL Accuracy, calculated as percentage recovery, was between 101.65% and 109.51%, with a RSD not higher than 0.41%. Topiramate is well resolved from others antiepileptic drugs and from the internal standard (Rs=5.20). In conclusion, the method is precise, accurate, reproducible and selective for the analysis of topiramate in human breast milk.