当社グループは 3,000 以上の世界的なカンファレンスシリーズ 米国、ヨーロッパ、世界中で毎年イベントが開催されます。 1,000 のより科学的な学会からの支援を受けたアジア および 700 以上の オープン アクセスを発行ジャーナルには 50,000 人以上の著名人が掲載されており、科学者が編集委員として名高い
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Akihiro Arakawa, Nobuaki Shiraki, Tomonori Tsuyama, Shoen Kume, Daigo Iwahata and Naoyuki Yamada
Zinc, iron and copper are essential mineral nutrients for the human body to function. It is also known that there are biological processes which relate to metals in the body including cells. Therefore, understanding about intracellular metals is important for the regeneration medicine. Intracellular metals were thought to be able to be divided into two forms by function: i) protein binding metals and ii) labile metals. However, it is difficult to quantify the concentration in both intracellular metal forms. In this study, the analytical method including the pre-treatment and HPLC coupled to ICP-MS (HPLC-ICP-MS) techniques was developed. Our method is able to fractionate two forms of intracellular metals and quantify each concentration, simultaneously. Finally, the presented method was applied to analysis of several cell states. As a result, dynamic changes of intracellular metal concentration were demonstrated during cell differentiation from iPS cells to pancreatic β cells.