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Terminal Restriction Fragment Length Polymorphism Analysis of Bacterial Population Difference in Mus Musculus Cecum in Response to Probiotic Administration

Leasha M. Christensen, Cynthia Blanton and Peter P. Sheridan

The cecum aids in maintaining homeostasis and host health by having the highest metabolite absorption and housing the most
abundant population of microbes in the mouse gastrointestinal tract. Various disease states, for which there is no standard medical
treatment, result from the disruption of the microbial populations in either presence or relative abundance due to various environmental
and host factors. Supplementation with probiotics, prebiotics, and synbiotics has shown promise as a therapeutic intervention to combat
dysbiosis. High throughput, culture-independent 16S rRNA terminal restriction length polymorphism analysis of cecum samples from
mice fed a control or synbiotic diet showed conservation of homeostatic balance in synbiotic-supplemented samples according to
Shannon, Simpson, and Margalef indices and insights into possible phylogenetics when further processed by Additive Main Effects and
Multiplicative Interactions analysis. Additional research is needed to more directly determine the diversity and phylogenetic effects of
synbiotic supplementation on cecum content for the alleviation of dysbiosis among the present microbial populations.