バイオテクノロジーとバイオマテリアル

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Thidiazuron Induces High Frequency Shoot Regeneration in Leaf and Petiole Explants of Cabbage (Brassica Oleracea L. Var. Capitata)

Geetika Gambhir and Srivastava DK

We have studied the effect of thidiazuron on shoot regeneration from leaf and petiole explants of cabbage (Brassica oleracea L. var. capitata cv. Pride of India). Different concentrations and combinations of thidiazuron (alone), thidiazuron+ Adenine, thidiazuron+ NAA and thidiazuron+ IAA were used in MS medium to optimize in vitro shoot regeneration from leaf and petiole explants of cabbage (Brassica Oleracea L. var. capitata cv. Pride of India). Leaf and petiole explants procured from 20-25 days old glasshouse grown seedlings of cabbage. Out of 40 combinations and concentrations tried separately for leaf and petiole explants, the high frequency shoot regeneration from leaf (91.11%) and petiole (88.88%) explant was obtained on MS medium supplemented with0.220mg/l thidiazuron + 0.02 mg/l NAA and 0.330 mg/l thidiazuron + 0.02 mg/l NAA respectively. High percentage root regeneration (100%) in in vitro developed shoots was obtained on MS medium supplemented with 0.10mg/l NAA. The regenerated complete plantlets were transferred to pots containing sterilized coco peat and acclimatized. A high efficiency and reliable plant regeneration protocol has been developed using thidiazuron. This regenerated protocol would be useful for genetic transformation studies in cabbage.